ABSTRACT
Objective To study and establish the extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L .) DC ..Methods Based on the singer factor test ,the influences of extracting times ,diameter of drug powder ,soaking time ,solid-liquid ratio ,ethanol concentration ,and extracting time on extraction ratio of total flavonoids were studied .The best extraction technology of total flavonoids from Gynura divaricata (L .) DC .was es-tablished through L9 (3)4 orthogonal tests .On the base of previous studies combine with L9 (3)4 orthogonal tests ,the best ex-traction technology of total polysaccharides from Gynura divaricata (L .) DC .was established .Results The optimized extrac-tion technology of total flavonoids was confirmed as follows :drug powder of less than 10 mesh diameter were soaking 0 .5 hour with 80% ethanol ,and solid-liquid ratio 1∶20 ,and extracting twice ,each time one hour .The optimized extraction technology of total polysaccharides was confirmed as follows :add 20 times water to the residue of ethanol ,extraction temperature 90℃ , and extracting twice ,each time one hour .The extraction rate of both total flavonoids and polysaccharides were more than 80% .Conclusion The extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L .) DC .established in this study is stable ,reproducible ,and lays a foundation for the further studies on hypogly-cemic components in Gynura divaricata (L .) DC .
ABSTRACT
<p><b>OBJECTIVE</b>To study the purification effect of macroporous resin combined with ZTC natural clarifying agent on Folium Gynurae Divaricatae extracts and to determine the ideal purification process.</p><p><b>METHOD</b>The content of total flavonoids in Folium Gynurae Divaricatae was determined by ultraviolet spectrophotometry spectrophotometer. The reservation ratio and transferring ratio of total flavonoids were used as indicator to detect such impacting factors as the macroporous resin model, the pH value of ethanol-extraction liquid and elution agent as well as cleaning agents, the concentration of ethanol-extraction liquid, the flow speed for adsorption, the ratio of polyamide to crude drug, the ratio of diameter to height of polyamide column, the flow speed of cleaning agents, the alcohol content and flow speed of the elution agent.</p><p><b>RESULT</b>The optimized purification conditions of total flavonoids in Folium Gynurae Divaricatae were as follows: the macroporous resin model was HPD600, the pH value of ethanol-extraction liquid and elution agent as well as cleaning agents was adjusted to 6.0, the concentration of extraction liquid was 0.25 g x mL(-1), the flow speed for adsorption was 2 BV x h(-1), the ratio of crude drug and the resin was 4:1, the ratio of diameter to height of resin column was 1:10, the flow speed of cleaning agents was 5 BV x h(-1), the dosage of cleaning agents was 5 BV, the dosage of elution agent was 9 BV, with 70% alcohol as elution agent, and the flow speed of elution agent as 5 BV x h(-1). Under the purification condition, the content of total flavonoids increased from 2.47% to 24.2%.</p><p><b>CONCLUSION</b>Macroporous resin and ZTC natural clarifying agent used in combination can improve the internal quality of the product, shorten the production cycle, reduce use and cost of organic solvent, thus it is worth popularizing.</p>